REAL-TIME NUCLEIC ACID AMPLIFICATION FLUORESCENCE DETECTION SYSTEM
- Good practicability
- Real-time data monitoring
- Real-time analysis display of the melting curve
- Quantitative analysis
- Product qualitation
- Automatic assessment of negative or positive result
- Wide detection scopes (101-1010copies/ml)
- High sensitivity and accuracy
TECHNICAL SPECIFICATION
Sample Capacity: 48 X 0.2ml
Excitation Light Sources: Light Emitting diode (LED).
Excitation optical wavelength: 470mm
Detection optical wavelength: 530mm
Detectable dyes: SYBR Green1, FAM, and TAMRA etc.
Sensitivity: 103 copies/ml
Reading time per sample: 1h20oC
Min reaction volume:20-50
Modular temperature scope: 30~99 oC
Temperature accuracy: ≤ +0.5
Temperature homogeneity: ≤ +0.5(after 30 seconds)
Rate of temperature ascending and descending: 3 oC/s (max).
Working environment, Environment temperature 10 ~ 30 oC and relative humidity: 20%~80%.
Inputing Power Supply: AC220V, 50Hz
Operating System: Window 2000/XP
Energy Consumption: 1200W
Overall Dimension: 520mm x 420 mm x 320mm (not including computer system).
Weight: 25 Kg (not including computer system).
REAL-TIME PCR
Polymerase chain reaction (PCR) is an important molecular diagnostic technique for the identification of infectious pathogens, viral load testing and the detection of inherited and acquired human genetic disorders.
PCR allows for the exponential amplification of a target fragment of DNA. The primers used are complementary to the target sequence of each of the DNA strands; DNA polymerase extends the primer to copy the designated sequence.
Real-Time PCR allows the monitoring of the PCR reaction in real time. This is measured by quantifying the fluorescence emitted from a specific reporter probe, which accumulates with the amplification of the PCR product. An increase in Reporter fluorescent signal is directly proportional to amplification of PCR product |
